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The A260/A280 ratio method estimates protein ________________ by measuring the absorbance maximum at 280nm caused by the amino acids___________________, ___________________, and ___________________. Since _______________ also absorbs in the UV range, we can correct for this contaminate by measuring the absorbance maximum at __________ and using the following equation:

Respuesta :

Answer:

Purity

Aromatic rings, peptide bonds, primary structure, secondary structure, and tertiary structure,

Nucleic acid,

260nm.

Explanation:

The A260/A280 ratio method estimates protein purity by measuring the absorbance maximum at 280nm caused by the amino acids aromatic rings, peptide bonds, primary structure, secondary structure, and tertiary structure. Since nucleic acid also absorbs in the UV range, we can correct for this contaminate by measuring the absorbance maximum at 260nm and using the following equation.

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